German
English
Friday, February 10, 2012
Abstracts from 
Fleischwirtschaft 90 (7), S. 96-99, 2010
Rapid detection of bacteria by biochips during food processing
By Bernd Heidenreich, Christopher Pöhlmann, Mathias Sprinzl und Manfred Gareis
biosensor | ribosomal RNA | detection | meat | E. coli
(original article is only in German language available)
According to EU hygiene guidelines, the control of microbiological criteria has to be tested throughout the whole process of food manufacturing. While classical microbiological methods are less suitable for routine purposes, biosensors integrated in lab-on-a-chip systems could help to improve the situation in future. Therefore, the applicability of
biosensors was tested for the detection of bacteria in food by means of direct and specific electrochemical detection of microbial 16S ribosomal RNA. E. coli and meat matrix was used in this study. An analysis procedure was developed including a short pre-enrichment step followed by an optimised RNA isolation and biochip measurement that
enabled E. coli detection of at least 1.cfu/ml sample within 7 hours. This diagnostic method is well suited for an application in monitoring food processing procedures.
Authors' addresses
Dr. Bernd Heidenreich, Unteres Lindig 5, 95494 Gesees; Dr. Christopher Pöhlmann,
Laboratorium für Biochemie, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth;
Prof. em. Dr. Dr. h. c. Mathias Sprinzl, Laboratorium für Biochemie, Universität Bayreuth,
Universitätsstraße 30, 95440 Bayreuth; Dir. u. Prof. Dr. Dr. Manfred Gareis, Institut für
Mikrobiologie und Biotechnologie des Max-Rubner-Instituts, E.-C.-Baumann-Straße 20,
95326 Kulmbach
According to EU hygiene guidelines, the control of microbiological criteria has to be tested throughout the whole process of food manufacturing. While classical microbiological methods are less suitable for routine purposes, biosensors integrated in lab-on-a-chip systems could help to improve the situation in future. Therefore, the applicability of
biosensors was tested for the detection of bacteria in food by means of direct and specific electrochemical detection of microbial 16S ribosomal RNA. E. coli and meat matrix was used in this study. An analysis procedure was developed including a short pre-enrichment step followed by an optimised RNA isolation and biochip measurement that
enabled E. coli detection of at least 1.cfu/ml sample within 7 hours. This diagnostic method is well suited for an application in monitoring food processing procedures.
Authors' addresses
Dr. Bernd Heidenreich, Unteres Lindig 5, 95494 Gesees; Dr. Christopher Pöhlmann,
Laboratorium für Biochemie, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth;
Prof. em. Dr. Dr. h. c. Mathias Sprinzl, Laboratorium für Biochemie, Universität Bayreuth,
Universitätsstraße 30, 95440 Bayreuth; Dir. u. Prof. Dr. Dr. Manfred Gareis, Institut für
Mikrobiologie und Biotechnologie des Max-Rubner-Instituts, E.-C.-Baumann-Straße 20,
95326 Kulmbach
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