German
English
Friday, July 30, 2010
Abstracts from 
Fleischwirtschaft 90 (1), S. 92-95, 2010
Detection of β-lactamase A and B in human and porcine strains of Yersinia enterocolitica 4/O:3 from Bavaria in Germany
By R. Bonke, C. Meyer, E. Stüber, M. Fredriksson-Ahomaa
β-lactamase | blaA | blaB | β-lactam-antibiotics |Y. enterocolitica 4/O:3 | agardiffusion | PCR
(original article is only in German language available)
Yersinia (Y.) enterocolitica 4/O:3 is one of the most important causesof food-borne yersiniosis in Germany. Pigs have been recognised as
the main reservoir for Y. enterocolitica bioserotype 4/O:3. Although
most of the clinical cases result in a self-limiting gastroenteritis in
humans, severe invasive disease including septicemia or reactive arthritis
can occur and may require antimicrobial therapy. Yersinia are
able to express β-lactamases, which can inactivate β-lactam-antibioticsand thus inhibit the antimicrobial treatment. One hundred strains of
Y. enterocolitica 4/O:3 from human and porcine origins from Bavaria
were tested by PCR and double-disk agar diffusion for the presence of
β-lactamase genes and the expression of β-lactamases. The β-lactamase
A gene was detected in 99% of the examined strains and the
β-lactamase B gene in all strains. All strains showed the expression of
β-lactamase A and 96% of the strains the expression of β-lactamase
B. Four human strains expressed no β-lactamase B although they exhibitthe β-lactamase B gene.
Authors' addresses
Dr. Rebecca Bonke, Dr. Cornelia Meyer, Dr. Elisabeth Stüber und Dr. Maria Fredriksson-
Ahomaa, Lehrstuhl für Lebensmittelhygiene, Veterinärwissenschaftliches Department,
Ludwig-Maximilians-Universität München, Schönleutnerstraße 8, 85764 Oberschleißheim,
Bonke@lmhyg.vetmed.uni-muenchen.de
Yersinia (Y.) enterocolitica 4/O:3 is one of the most important causesof food-borne yersiniosis in Germany. Pigs have been recognised as
the main reservoir for Y. enterocolitica bioserotype 4/O:3. Although
most of the clinical cases result in a self-limiting gastroenteritis in
humans, severe invasive disease including septicemia or reactive arthritis
can occur and may require antimicrobial therapy. Yersinia are
able to express β-lactamases, which can inactivate β-lactam-antibioticsand thus inhibit the antimicrobial treatment. One hundred strains of
Y. enterocolitica 4/O:3 from human and porcine origins from Bavaria
were tested by PCR and double-disk agar diffusion for the presence of
β-lactamase genes and the expression of β-lactamases. The β-lactamase
A gene was detected in 99% of the examined strains and the
β-lactamase B gene in all strains. All strains showed the expression of
β-lactamase A and 96% of the strains the expression of β-lactamase
B. Four human strains expressed no β-lactamase B although they exhibitthe β-lactamase B gene.
Authors' addresses
Dr. Rebecca Bonke, Dr. Cornelia Meyer, Dr. Elisabeth Stüber und Dr. Maria Fredriksson-
Ahomaa, Lehrstuhl für Lebensmittelhygiene, Veterinärwissenschaftliches Department,
Ludwig-Maximilians-Universität München, Schönleutnerstraße 8, 85764 Oberschleißheim,
Bonke@lmhyg.vetmed.uni-muenchen.de
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